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  1. #11
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    Hi All,

    Some Augment results from the Toronto and Turkey clinics are going to be presented at a conference. I found the abstracts here:

    http://www.sri-online.org/UserFiles/...s_LB_Final.pdf

    (Searching for Casper and Oktay in the document will find the two abstracts)

    Here is the first one:

    LB-002
    Preliminary Results with Autologous Egg Precursor Cell Mitochondrial Injection during Intracytoplasmic Sperm Injection (ICSI) in Women with Previous Poor Embryo Development. Robert F Casper, Dennis B Dela Cruz, Frederic Mitri, Anat Hershko, Yaakov Bentov, Paul Chang, Navid Esfandiari. University of Toronto, Toronto, ON, Canada.
    INTRODUCTION: As women age, oocyte mitochondrial activity declines leading to lower ATP levels and less likelihood of blastocyst development. In animal and human studies, the transfer of young donor ooplasm including mitochondria into oocytes previously producing abnormal embryos has led to improved embryo development and pregnancy. Unfortunately, these procedures result in mitochondrial heteroplasmy. Discovery of egg precursor cells in the ovarian cortex now allows preparation of potentially healthy autologous mitochondria for injection without creating heteroplasmy.
    METHODS: We evaluated our preliminary clinical experience in treating 24 women under age 40 who had ≥ one failed IVF cycle with poor embryo quality including fragmentation and arrest. Patients had laparoscopic ovarian cortex biopsy with scissors and egg precursor cells were identified using flow cytometry with a specific antibody and cryopreserved. On the day of oocyte retrieval for IVF, the egg precursor cells were thawed, and cell membranes disrupted to obtain an enriched mitochondrial preparation.
    Approximately1-2 pL of mitochondria were injected into MII oocytes together with a spermatozoon at the time of ICSI.
    RESULTS: Two cycles were not completed; one because of no oocytes retrieved and one with all oocytes vitrified because no sperm could be obtained. Of 22 treated cycles there were 3 cycles with no normal fertilization, 3 with arrested embryo development, and 3 in which all blastocysts were vitrified (2 because of OHSS risk, and 1 because of thin endometrium). The remaining thirteen women completed cycles with embryo transfer (ET) including 3 day 3 transfers (poor embryo quality, 0 pregnant) and 10 blastocyst transfers (6 pregnant). Therefore, there were 6 clinical pregnancies in 13 ET cycles (46%) in women with previous poor embryo development.There was one spontaneous abortion at 6 weeks.
    CONCLUSIONS: Our clinical experience with autologous egg precursor cell mitochondrial injections at the time of ICSI appear promising in women with previously poor embryo development and failed IVF cycles. Consequently, rather than moving to donor oocytes, it may be possible to obtain live births with a patient’s own oocytes. In addition, further research in our lab is exploring whether this procedure may be beneficial for women with failed IVF cycles over the age of 40 years.

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    Golden Egg  (09-04-2015)

  3. #12
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    Here is the second one:
    LB-044
    Oogonial Precursor Cell Derived Autologous Mitochondria Injection Improves Outcomes in Women with Multiple IVF
    Failures Due to Low Oocyte Quality. Kutluk Oktay,1,3 Murat Sonmezer,2
    Volkan Baltaci,3 Evrim Unsal,3 Suleyman Aktuna,3 Aysun Baltaci,3
    Volkan Turan,3 Murat Seval.2 1New York Medical College, Valhalla, NY, USA; 2Ankara University, Ankara, Turkey; 3Genart, Ankara, Turkey.
    INTRODUCTION: Mitochondrial dysfunction has been suggested as a major cause of age-induced decline in oocyte quality. In the past, cytoplasmic transfer from donor oocytes to aged oocytes showed some success but was abandoned due to the concerns with heteroplasmy. Recent studies indicated presence of oogonial precursor cells (OPC) in the human ovary, which could be an autologous source of “healthy mitochondria”. Our objective was to investigate the clinical efficacy of OPC-derived autologous mitochondrial
    injection (AMI) to improve oocyte quality.
    METHODS: Laparoscopic ovarian cortical biopsies were obtained from 8 women aged 27-41 (mean 34.6±4.5) with ≥3 IVF failures
    and poor oocyte/embryo quality.OPCs were isolated by cell sorting using a proprietary monoclonal anti-DDX antibody. OPCs were
    then disrupted and mitochondria were isolated. Reconstituted mitochondria were injected into each oocyte during ICSI. Paired comparisons were made between the first as well as the mean of all failed cycles and the post AMI cycle.
    RESULTS: Patients were older as compared to when they had failed IVF cycles and, expectedly produced fewer oocytes post-AMI. The fertilization rates improved significantly post AMI, however, the improvement in embryo grading was not statistically significant.

    n=8 Pre-AMI:1st cycle(a) Pre-AMI:All cycles(b) Post-AMI(c) P (a vs c) P (b vs c)
    Age 29.8±5.8 3 1.1±5.4 34.6±4.5 0.01 0.01
    N of oocytes 14.2±5.4 12.5±5.5 7. 7±3.1 0.03 0.03
    N of mature oocytes 11.3±4.9 10.8±4.9 6.1±3.2 0.01 0.01
    2PN embryos 6.0±5.8 5.6±4.4 4.2±1.9 0.39 0.31
    Fertilization rate (%) 48.3±36. 3 51.5±27.0 77.5±21.1 0.06 0.03
    D3 embryo grade 1.95±1.29 1.71±0.57 1.56±0.46 0.24 0.34

    Of the 8 embryo transfers, 2 resulted in a conception, with an ongoing pregnancy rate of 25%. Interestingly, both pregnancies were from single frozen embryo transfers after 4 and 7 IVF failures; one had been tested by aCGH and found to be euploid.
    CONCLUSIONS: These data show encouraging results for OPC-derived AMI, despite the older age at the time of the treatment when compared to the previous failed IVF cycles. These include a significant improvement in fertilization rates, and pregnancies after single embryo transfers.

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    Golden Egg  (09-04-2015)

  5. #13
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    Incredibly exciting! I've been keeping up with the Inspire forum with great interest.

  6. #14
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    New presentation coming soon ladies! They are reporting some results from Augment. Text copied from the Ovascience website is below:

    ----------------------------------
    OvaScience to Host Conference Call and Webcast Presentation with IVF Specialists to Review Early AUGMENT Fertility Treatment Patient Experience
    Overview of AUGMENT Presentation at Society for Reproductive Investigation Meeting and Select Patient Case Studies
    CAMBRIDGE, Mass.--(BUSINESS WIRE)--Mar. 25, 2015-- OvaScienceSM (NASDAQ: OVAS), a global fertility company focused on the discovery, development and commercialization of new treatment options, announced today a conference call focused on the AUGMENTSM treatment on Friday, March 27, 2015 at 10:00 am ET. The presentation will follow an AUGMENT treatment presentation at the Society for Reproductive Investigation (SRI) 62nd Annual Scientific Meeting, which represents the first time detailed clinical experience will be presented.

    Company management will be joined by two leading in vitro fertilization (IVF) specialists among the first to use the AUGMENT treatment in clinical practice.
    Robert Casper, M.D., F.R.C.S.(C), Medical Director of TCART Fertility Partners in Toronto, Canada, and Kutluk Oktay, M.D., F.A.C.O.G, of Gen-art IVF in Ankara, Turkey, will present initial AUGMENT experience from their respective clinics, including select patient case studies. The AUGMENT treatment is not available in the United States.
    A live, listen-only presentation can be accessed via webcast by visiting the Investors section of the Company’s website at www.ovascience.com. The call can be accessed by dialing (877) 930-8299 (U.S.) or (253) 336-8765 (international) five minutes prior to the start of the call and providing the passcode 14733732. A replay of the webcast will be archived on the Company’s website for two weeks following the event.

  7. The Following User Says Thank You to chook11 For This Useful Post:

    Golden Egg  (09-04-2015)

  8. #15
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    Also, finally got AF yesterday - so not completely in menopause yet.

  9. #16
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    Chook, that's great news re your AF arriving.

  10. #17
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    First Baby Born with OvaScience’s AUGMENT Fertility Treatment

    http://ir.ovascience.com/phoenix.zht...cle&ID=2045382

  11. #18
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    Hi chook11,

    Did you read the recent inspiring news First Augment Baby bone in April 2015.

    http://time.com/3849127/baby-stem-cells-augment-ivf/

    I am at similar situation as you, I have no egg, so I cannot do Augment, I am also anxiously waiting for OvaPrime, hope it can help.

    I also have contacted TCART and First Steps Fertility in Canada, hopefully I can be the candidates to receive treatment.

    Hopefully we can share information we have.

    Good luck!

  12. #19
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    Hi Blueberry,

    Great to hear from you. Did you get an email back from Caroline at TCART?

    There is a new presentation available on the Ovascience website:
    http://wsw.com/webcast/jeff88/ovas/

    best wishes,
    Chook

  13. #20
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    Hi Chook,

    Nice to hear from you too. I wrote to all 4 Augment clinics, and got response ftom each but not TCART. Anyway, First Step Fertility told me Augment does not suit me, but OvaPrime should be able to help, they will keep me posted for OvaPrime.

    I read your previous threads, you did not have period for a while until March 2015. I had similar situation last year, no period for almost 3 months, I then went to see Chinese medicine doctor, started to have acupunture and herbal medicine. 6 weeks after I got my period, I then continued acupunture and herbal medicine, since then my period came every month. So, would recommend you try acupuncture and herbal medicine if you still have problem.

    Did you try mini-IVF? My doctor is doing mini-IVF for me now. I am 45 yo, high dosage Gonf does not work. Hope I can have luck soon.

    Kind regards,
    Blueberry70


 

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  1. OvaScience
    By chook11 in forum IVF
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    Last Post: 18-12-2014, 05:25

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