Some Augment results from the Toronto and Turkey clinics are going to be presented at a conference. I found the abstracts here:
(Searching for Casper and Oktay in the document will find the two abstracts)
Here is the first one:
Preliminary Results with Autologous Egg Precursor Cell Mitochondrial Injection during Intracytoplasmic Sperm Injection (ICSI) in Women with Previous Poor Embryo Development. Robert F Casper, Dennis B Dela Cruz, Frederic Mitri, Anat Hershko, Yaakov Bentov, Paul Chang, Navid Esfandiari. University of Toronto, Toronto, ON, Canada.
INTRODUCTION: As women age, oocyte mitochondrial activity declines leading to lower ATP levels and less likelihood of blastocyst development. In animal and human studies, the transfer of young donor ooplasm including mitochondria into oocytes previously producing abnormal embryos has led to improved embryo development and pregnancy. Unfortunately, these procedures result in mitochondrial heteroplasmy. Discovery of egg precursor cells in the ovarian cortex now allows preparation of potentially healthy autologous mitochondria for injection without creating heteroplasmy.
METHODS: We evaluated our preliminary clinical experience in treating 24 women under age 40 who had ≥ one failed IVF cycle with poor embryo quality including fragmentation and arrest. Patients had laparoscopic ovarian cortex biopsy with scissors and egg precursor cells were identified using flow cytometry with a specific antibody and cryopreserved. On the day of oocyte retrieval for IVF, the egg precursor cells were thawed, and cell membranes disrupted to obtain an enriched mitochondrial preparation.
Approximately1-2 pL of mitochondria were injected into MII oocytes together with a spermatozoon at the time of ICSI.
RESULTS: Two cycles were not completed; one because of no oocytes retrieved and one with all oocytes vitrified because no sperm could be obtained. Of 22 treated cycles there were 3 cycles with no normal fertilization, 3 with arrested embryo development, and 3 in which all blastocysts were vitrified (2 because of OHSS risk, and 1 because of thin endometrium). The remaining thirteen women completed cycles with embryo transfer (ET) including 3 day 3 transfers (poor embryo quality, 0 pregnant) and 10 blastocyst transfers (6 pregnant). Therefore, there were 6 clinical pregnancies in 13 ET cycles (46%) in women with previous poor embryo development.There was one spontaneous abortion at 6 weeks.
CONCLUSIONS: Our clinical experience with autologous egg precursor cell mitochondrial injections at the time of ICSI appear promising in women with previously poor embryo development and failed IVF cycles. Consequently, rather than moving to donor oocytes, it may be possible to obtain live births with a patient’s own oocytes. In addition, further research in our lab is exploring whether this procedure may be beneficial for women with failed IVF cycles over the age of 40 years.