As a general rule, I think your house, your rules. But I don't have one iota of concern over germs and floors. Germs are fricken everywhere - if I let myself worry about stuff like that I'd end up bat$hit crazy.
I'm pretty sure I read somewhere that mobile phones have more bacteria on them than a toilet seat.
Quick, get the antibacterial wipes out!
Oh this is ridiculous but entertaining. If you don't want people wearing shoes in your house, tell them they have to take them off. Kids will be exposed to germs no matter what you do. Are they not allowed to play at the park because other kids are wearing shoes that have touched a toilet floor and then walked on the equipment? I don't think removing shoes will affect a child's health but if you're not comfortable with it, just make your house a shoes off house. It doesn't need to be an issue.
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