I think mine was about a week after ovulation. I was breastfeeding my DS and DD was my first ovulation. I would not have had a clue if my dog did not start attacking everything that got near me, only because that is what she did the previous time. I went for bloods, not realy thinking i was prg and the results came back inconclusive. My doc said, well you have prg hormones but not enough for him to confirm and say i was prg, i had to go back 3 days later and it was confirmed after a weekend of torture.