Hi Beck - I don't know if Pregnyl is the same as the Trigger injection we use, but we were getting absolutely no second line at 5 days post transfer (so all HCG was out of Kate's body) and the second line only appeared after 7 days post transfer. I can't imagine something staying in your system for so long. Plus if the second line is darker today than yesterday I am sure you are pregnant. Don't beat yourself up lovely - it is such a trying journey and so hard to be patient. The FN's just like to be extra cautious. Stay positive and keep doing your tests each day. If you are not pregnant then that line should be getting lighter, not darker. Can you ask for a pregnancy test tomorrow? Instead of waiting for Monday? If you are past your due date then surely they can let you get a blood test just for your peace of mind.